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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
28/03/2011 |
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Data da última atualização: |
04/04/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HANISCH, A. L.; MARQUES, A. C.; BONA, L. C. |
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Afiliação: |
Epagri |
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Título: |
Resposta de pastagens nativas adubao com insumos agroecolgicos em reas de cava no Planalto Norte Catarinense. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Revista de Estudos Vale do Iguaçu, União da Vitória, n. 14, p. 139-148, 2009. |
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Idioma: |
Português |
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Conteúdo: |
Foi conduzida uma pesquisa na região do Planalto Norte Catarinense, entre outubro de 2007 e fevereiro de 2008, objetivando avaliar, de forma participativa, o efeito do uso de insumos agroecológico sobre pastagens nativas em áreas de caíva. Foi utilizado delineamento em blocos casualizados com três repetições e quatro tratamentos (dose t ha-1): 1) Calcário (2) + cama de aviário (2) + fosfato natural (0,8); 2) fosfato natural (0,8); 3) pó de basalto (2); 4) Testemunha. Foi avaliada a produção de matéria seca (PRODMS) e teores de proteína bruta (PB) e digestibilidade (DIVMO) das pastagens. A área foi utilizada por rebanho leiteiro em pastejo rotativo a cada 28 dias. Não houve diferença significativa (P>0,05) entre os tratamentos com insumos, mas todos diferiram da testemunha. A PRODMS variou de 5.244 a 2.317 kg ha-1 de MS, respectivamente para o uso de pó de basalto e para a testemunha. Os teores médios de PB e DIVMO da pastagem foram respectivamente de 12,9% e 61,6%. A pastagem era composta predominantemente de espécies dos gêneros Axonopus, Paspalum e Desmodium. |
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Palavras-Chave: |
Agroecologia; Fosfato natural; Pó de basalto; Silvipastoril. |
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Categoria do assunto: |
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LEADER 01628naa a2200169 a 4500
001 1075163
005 2011-04-04
008 2009 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aResposta de pastagens nativas adubao com insumos agroecolgicos em reas de cava no Planalto Norte Catarinense.
260 $c2009
520 $aFoi conduzida uma pesquisa na região do Planalto Norte Catarinense, entre outubro de 2007 e fevereiro de 2008, objetivando avaliar, de forma participativa, o efeito do uso de insumos agroecológico sobre pastagens nativas em áreas de caíva. Foi utilizado delineamento em blocos casualizados com três repetições e quatro tratamentos (dose t ha-1): 1) Calcário (2) + cama de aviário (2) + fosfato natural (0,8); 2) fosfato natural (0,8); 3) pó de basalto (2); 4) Testemunha. Foi avaliada a produção de matéria seca (PRODMS) e teores de proteína bruta (PB) e digestibilidade (DIVMO) das pastagens. A área foi utilizada por rebanho leiteiro em pastejo rotativo a cada 28 dias. Não houve diferença significativa (P>0,05) entre os tratamentos com insumos, mas todos diferiram da testemunha. A PRODMS variou de 5.244 a 2.317 kg ha-1 de MS, respectivamente para o uso de pó de basalto e para a testemunha. Os teores médios de PB e DIVMO da pastagem foram respectivamente de 12,9% e 61,6%. A pastagem era composta predominantemente de espécies dos gêneros Axonopus, Paspalum e Desmodium.
653 $aAgroecologia
653 $aFosfato natural
653 $aPó de basalto
653 $aSilvipastoril
773 $tRevista de Estudos Vale do Iguaçu, União da Vitória$gn. 14, p. 139-148, 2009.
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Epagri-Sede (Epagri-Sede) |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
17/08/2021 |
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Data da última atualização: |
17/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - B |
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Autoria: |
ARAÚJO, E. R.; RESENDE, R. S.; LIMA, M. F. |
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Título: |
First report of Iris yellow spot orthotospovirus infecting onion in Santa Catarina State, Brazil. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 47, n. 2, p. 131-133, 2021. |
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Idioma: |
Português |
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Conteúdo: |
Onion (Allium cepa L.) is the third most economically important vegetable crop in Brazil; its planted area was estimated at approximately 48,000 hectares with total production of 1,556,885 tons in 2019. Santa Catarina is the largest national producing state, accounting for 532,000 tons in the 2019/20 crop season. Iris yellow spot orthotospovirus (IYSV), genus Orthotospovirus, family Tospoviridae, is transmitted by Thrips tabaci L. in a persistent and propagative manner. Frankliniella fusca can also transmit IYSV but less efficiently. IYSV has been reported in South, Central and North America, Africa, Europe, Asia, and Oceania. In Brazil, the first report of IYSV-like symptoms in onion fields occurred in 1981, in Rio Grande do Sul State, and the causal agent was initially identified as tomato spotted wilt-like virus. However, correct identification of the pathogen as IYSV, causing the disease known as ?sapeca?, was obtained only in 1994, when disease symptoms were observed in onion fields in the sub-middle region of São Francisco River Valley ? Pernambuco and Bahia States. Since the 1990s, no new reports on the occurrence of IYSV infecting onion fields in the country have been made. During 2017/18, 2018/19 and 2019/20 crop seasons, sudden leaf blight symptoms were observed in bulb onion crops in open fields in Santa Catarina State. Eventually, leaves exhibited whitish diamond-shaped spots, characteristic of IYSV-like symptoms. In turn, yellow or light green diamond-shaped spots, which may or may not have a lighter center, were observed on scape from seed onion crop. Sample collection was performed considering three crop seasons: 2017/18: 63 leaf samples were collected ? 56 from symptomatic onions and seven from weeds showing leaf spots [Bidens pilosa (three samples), Amaranthus sp. (two samples), Raphanus sp. (one sample), and Euphorbia heterophylla (one sample)]; 2018/19: 12 leaf samples were collected ? seven from symptomatic and five from asymptomatic onion plants; 2019/20: 30 leaf samples were collected ? all from symptomatic onion plants. Samples were evaluated by NCMELISA (Nitrocellulose Membrane-enzyme-linked immunosorbent assay) with polyclonal antibodies raised against the nucleoprotein of IYSV (produced at the National Center for Vegetable Crops Research ? CNPH) or using IYSV ImmunoStrips (Agdia Inc., Elkhart, IN) or even by DAS-ELISA (Double Antibody Sandwich enzyme-linked immunosorbent assay), according to the manufacturer?s instructions (Agdia Inc., Elkhart, IN). For each serological test, the sample was considered positive, or virus-infected, compared to the reaction of the positive control (NCM-ELISA: formation of precipitate by the action of enzymatic reaction on the BCIP and NBT, resulting in purplish spots; ImmunoStrips: appearance of a dark line in the positive control; DAS-ELISA: absorbance reading of the sample was at least twice the mean absorbance value recorded for the extract of a healthy plant used as negative control). Out of 105 field samples analyzed, 33 (31.4%) had a positive signal to IYSV antibodies, compared to the negative and IYSV-positive controls. It is worth pointing out that all IYSV-positive samples were collected from symptomatic onion plants. Besides, no leaf samples obtained from symptomless onion plants or weed species tested positive. IYSV has been detected in six counties in Santa Catarina State. Interestingly, samples collected in all three crop seasons led to similar infection rates. Among IYSV-positive samples, 20 were collected in the 2017/18 crop season (11 from Ituporanga, five from Leoberto Leal, one from Alfredo Wagner, one from Aurora, one from Atalanta, and one from Canoinhas) and showed an infection rate of 31.74%. Leaf samples collected in the 2018/19 crop season also tested positive for IYSV (two from Aurora and two from Ituporanga), resulting in an infection rate of 33.33%. Nine samples from the last crop season (2019/2020) also reacted positively with IYSV antibodies (all from Ituporanga County), producing an infection rate of 30.0%. Considering that the collection of field samples focused on symptomatic plants, the overall detection rate (31.4%) can be regarded as relatively low; however, possible justification for symptomatic samples that tested negative to specific IYSV polyclonal antibodies (68.6%) are: i) The plants were not infected with IYSV and the observed symptoms were due to other causes, such as abiotic factors, since not all of them had diamond-shaped lesions, a characteristic symptom of this disease. ii) Sampling: differently from other orthotospovirus species, IYSV moves less systemically in the infected plant and tends to cause localized infection. The virus does not present an even distribution in the infected plant, and high virus titers are usually found for inner leaves. In addition, the virus may not be found in all leaves. IYSV tends to be localized in and in close proximity to the lesions on leaves and scapes. It is frequently detected in visible lesions of at least 30-50 mm. Thus, the portion of the leaf sample used for serological analysis could not contain enough viral particles for detection by ELISA, which may eventually result in false negatives. iii) The plants could be infected with other viruses and not with IYSV. All positive and negative controls used in the serological tests (NCM-ELISA; DAS-ELISA) reacted as expected. IYSV may become an emerging virus to onion crops in Brazil as it is in other regions of the world. Monitoring onion fields is mandatory to learn about the geographical distribution, spread and occurrence of this disease. To our knowledge, this is the first report of Iris yellow spot orthotospovirus infecting onion in Santa Catarina State. MenosOnion (Allium cepa L.) is the third most economically important vegetable crop in Brazil; its planted area was estimated at approximately 48,000 hectares with total production of 1,556,885 tons in 2019. Santa Catarina is the largest national producing state, accounting for 532,000 tons in the 2019/20 crop season. Iris yellow spot orthotospovirus (IYSV), genus Orthotospovirus, family Tospoviridae, is transmitted by Thrips tabaci L. in a persistent and propagative manner. Frankliniella fusca can also transmit IYSV but less efficiently. IYSV has been reported in South, Central and North America, Africa, Europe, Asia, and Oceania. In Brazil, the first report of IYSV-like symptoms in onion fields occurred in 1981, in Rio Grande do Sul State, and the causal agent was initially identified as tomato spotted wilt-like virus. However, correct identification of the pathogen as IYSV, causing the disease known as ?sapeca?, was obtained only in 1994, when disease symptoms were observed in onion fields in the sub-middle region of São Francisco River Valley ? Pernambuco and Bahia States. Since the 1990s, no new reports on the occurrence of IYSV infecting onion fields in the country have been made. During 2017/18, 2018/19 and 2019/20 crop seasons, sudden leaf blight symptoms were observed in bulb onion crops in open fields in Santa Catarina State. Eventually, leaves exhibited whitish diamond-shaped spots, characteristic of IYSV-like symptoms. In turn, yellow or light green diamond-shaped spo... Mostrar Tudo |
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Palavras-Chave: |
Allium cepa; ocorrência; virose. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 06301naa a2200181 a 4500
001 1131108
005 2021-08-17
008 2021 bl uuuu u00u1 u #d
100 1 $aARAÚJO, E. R.
245 $aFirst report of Iris yellow spot orthotospovirus infecting onion in Santa Catarina State, Brazil.$h[electronic resource]
260 $c2021
520 $aOnion (Allium cepa L.) is the third most economically important vegetable crop in Brazil; its planted area was estimated at approximately 48,000 hectares with total production of 1,556,885 tons in 2019. Santa Catarina is the largest national producing state, accounting for 532,000 tons in the 2019/20 crop season. Iris yellow spot orthotospovirus (IYSV), genus Orthotospovirus, family Tospoviridae, is transmitted by Thrips tabaci L. in a persistent and propagative manner. Frankliniella fusca can also transmit IYSV but less efficiently. IYSV has been reported in South, Central and North America, Africa, Europe, Asia, and Oceania. In Brazil, the first report of IYSV-like symptoms in onion fields occurred in 1981, in Rio Grande do Sul State, and the causal agent was initially identified as tomato spotted wilt-like virus. However, correct identification of the pathogen as IYSV, causing the disease known as ?sapeca?, was obtained only in 1994, when disease symptoms were observed in onion fields in the sub-middle region of São Francisco River Valley ? Pernambuco and Bahia States. Since the 1990s, no new reports on the occurrence of IYSV infecting onion fields in the country have been made. During 2017/18, 2018/19 and 2019/20 crop seasons, sudden leaf blight symptoms were observed in bulb onion crops in open fields in Santa Catarina State. Eventually, leaves exhibited whitish diamond-shaped spots, characteristic of IYSV-like symptoms. In turn, yellow or light green diamond-shaped spots, which may or may not have a lighter center, were observed on scape from seed onion crop. Sample collection was performed considering three crop seasons: 2017/18: 63 leaf samples were collected ? 56 from symptomatic onions and seven from weeds showing leaf spots [Bidens pilosa (three samples), Amaranthus sp. (two samples), Raphanus sp. (one sample), and Euphorbia heterophylla (one sample)]; 2018/19: 12 leaf samples were collected ? seven from symptomatic and five from asymptomatic onion plants; 2019/20: 30 leaf samples were collected ? all from symptomatic onion plants. Samples were evaluated by NCMELISA (Nitrocellulose Membrane-enzyme-linked immunosorbent assay) with polyclonal antibodies raised against the nucleoprotein of IYSV (produced at the National Center for Vegetable Crops Research ? CNPH) or using IYSV ImmunoStrips (Agdia Inc., Elkhart, IN) or even by DAS-ELISA (Double Antibody Sandwich enzyme-linked immunosorbent assay), according to the manufacturer?s instructions (Agdia Inc., Elkhart, IN). For each serological test, the sample was considered positive, or virus-infected, compared to the reaction of the positive control (NCM-ELISA: formation of precipitate by the action of enzymatic reaction on the BCIP and NBT, resulting in purplish spots; ImmunoStrips: appearance of a dark line in the positive control; DAS-ELISA: absorbance reading of the sample was at least twice the mean absorbance value recorded for the extract of a healthy plant used as negative control). Out of 105 field samples analyzed, 33 (31.4%) had a positive signal to IYSV antibodies, compared to the negative and IYSV-positive controls. It is worth pointing out that all IYSV-positive samples were collected from symptomatic onion plants. Besides, no leaf samples obtained from symptomless onion plants or weed species tested positive. IYSV has been detected in six counties in Santa Catarina State. Interestingly, samples collected in all three crop seasons led to similar infection rates. Among IYSV-positive samples, 20 were collected in the 2017/18 crop season (11 from Ituporanga, five from Leoberto Leal, one from Alfredo Wagner, one from Aurora, one from Atalanta, and one from Canoinhas) and showed an infection rate of 31.74%. Leaf samples collected in the 2018/19 crop season also tested positive for IYSV (two from Aurora and two from Ituporanga), resulting in an infection rate of 33.33%. Nine samples from the last crop season (2019/2020) also reacted positively with IYSV antibodies (all from Ituporanga County), producing an infection rate of 30.0%. Considering that the collection of field samples focused on symptomatic plants, the overall detection rate (31.4%) can be regarded as relatively low; however, possible justification for symptomatic samples that tested negative to specific IYSV polyclonal antibodies (68.6%) are: i) The plants were not infected with IYSV and the observed symptoms were due to other causes, such as abiotic factors, since not all of them had diamond-shaped lesions, a characteristic symptom of this disease. ii) Sampling: differently from other orthotospovirus species, IYSV moves less systemically in the infected plant and tends to cause localized infection. The virus does not present an even distribution in the infected plant, and high virus titers are usually found for inner leaves. In addition, the virus may not be found in all leaves. IYSV tends to be localized in and in close proximity to the lesions on leaves and scapes. It is frequently detected in visible lesions of at least 30-50 mm. Thus, the portion of the leaf sample used for serological analysis could not contain enough viral particles for detection by ELISA, which may eventually result in false negatives. iii) The plants could be infected with other viruses and not with IYSV. All positive and negative controls used in the serological tests (NCM-ELISA; DAS-ELISA) reacted as expected. IYSV may become an emerging virus to onion crops in Brazil as it is in other regions of the world. Monitoring onion fields is mandatory to learn about the geographical distribution, spread and occurrence of this disease. To our knowledge, this is the first report of Iris yellow spot orthotospovirus infecting onion in Santa Catarina State.
653 $aAllium cepa
653 $aocorrência
653 $avirose
700 1 $aRESENDE, R. S.
700 1 $aLIMA, M. F.
773 $tSumma Phytopathologica, Botucatu$gv. 47, n. 2, p. 131-133, 2021.
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